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Seed DNA Extractor The DNA extracted from the dried seeds was detected by 1% agarose gel electrophoresis. The DNA was intact and there was no residue in the RNA. This indicated that the dry seeds used this method to extract DNA. The experiment used the embryo-free half-grain barley seeds to extract DNA. Compared with the DNA extracted from the leaves, the results showed that the DNA extracted from the dried seeds is the same size as the leaves extracted from the leaves. Therefore, it is reliable to extract the DNA from the dried seeds of the barley. of. In addition, semi-seeds with embryos can also be used for morphological observations, both for DNA detection and phenotypic traits for barley.
The material was removed from the seed coat and the seeds were cut transversely in the middle. If it is necessary to continue morphological observation using the portion with endosperm, the endosperm cannot be damaged when the seeds are cut. The peeled embryo-free half-seeds (30-50 mg) were clamped into 2.0-ml centrifuge tubes with forceps, liquid nitrogen was added, and quickly ground into a white powder with an electric drill (glass drill) (due to the small sample size, Can not be ground in the mortar, or can not be completely collected), operate or placed in -80 °C for the extraction of seed DNA extractor spare.
Samples extracted from seed DNA extractors were used to analyze high levels of protein, sugar and RNA in dried barley seeds. If the removal of macromolecules and other substances is incomplete, DNA is encapsulated by these macromolecules and it is difficult to dissolve in TE. Subsequent research has a great influence. Therefore, in the experiment, when the DAN was precipitated, the concentration of 3 mol/L sodium acetate was added to mix and then isopropyl alcohol was added. Thus, although the DNA was slightly lost, the purification effect was good.
Seed DNA Extractor Used for Detecting the Character of Dried Barley Seeds
The study of genetic characteristics of plants in the research of seed plants, whether in agriculture or in forestry, is an important issue. The study of natural genetic properties can not be separated from the arrangement of DNA extraction and the composition of the components of DNA extraction. The extraction of DNA in plants basically uses plant tissues, especially leaves, and it is also true that the research in such seeds has become more and more important to scholars. The extraction of DNA from barley seeds by seed DNA extraction instrumentation can provide a certain reference for extraction.